The following table shows some of the Watson
Standard tube lengths during the years when this was noted in the
catalogs. Watson usually noted that they could supply objectives
designed for any tube length desired by special order but usually at no
additional charge.
The 160 mm length, eventually the RMS standard,
was usually readily available also because it was the Continental
standard for many years.
YEAR | WATSON STANDARD TUBE LENGTH(S) |
1906 |
TEN INCHES (250 MM) |
1912-13 |
EIGHT INCHES (200 MM) |
1918 |
TEN INCHES (250 MM) |
1923 |
TEN INCHES (250 MM) AND 170 MM (6.7 INCHES, LIETZ STANDARD) |
1928 |
160 MM ( 6.3 INCHES, RMS STANDARD) |
SOME NOTES ABOUT TUBE LENGTH, FOCAL LENGTH, MAGNIFICATION, AND OPTICAL PERFORMANCE
The reader, and even some modern experienced microscopists, may be
confused about tube lengths and magnification vs focal length (FL).
First one must understand that there are two different types of tube
length; the one alluded to above is the mechanical tube
length-the distance from the top of the tube to the bottom where the
objective screws in. The second and more difficult to understand is the
optical tube length (OTL). This is defined as the distance from
the back focal plane of the objective (usually located somewhere in the
middle of the objective itself), and the primary image plane within the
eyepiece, usually about a cm below the top of the optical tube. This
distance varies not only with the focal length of the objective, but
also varies with its design; in other words the OTL with identical
mechanical tube length and identical eyepieces but with a 6 mm Parachromatic objective will not be the same as the exact same setup but with a 6 mm Apochromat or a 6 mm Holos.
Generally magnification of the objective is equal to the OTL/FL of the
objective. To make matters worse the exact magnification will vary from
person to person as each of our own eyes have different degrees of
distortion from 'ideal', i.e. we are all far-sighted or near sighted to
some degree. Using spectacles changes the equation again so even if we
wear corrective lenses, these changes in magnification cannot be
predicted without direct measurement of all the factors involved.
Suffice to say that the modern practice of labelling objectives with a
magnification power is a bit misleading as the exact magnification is
unlikely to be that exact number! Lastly, consider that ordinary
objectives are designed for a standard coverslip thickness of either
0.17 (presently) or 0.18 mm in thickness (previously); any deviation in
the distance of the specimen from the top of the coverslip will have an
increasing effect of degrading the quality of the image as the
magnification increases or the focal length decreases. It is for this
reason that with higher powers, a correction collar or drawtube are
needed to allow adjustment of tube length to suit any variation in the
coverslip-top-to-specimen distance. For this reason, critical mounting
is sometimes done on the underside of a coverslip of standard
thickness, and then the mount cemented to the slide. It is because
critical adjustments to tube length may be needed at high power, that
the mechanically adjustable drawtube of the Van Heurck microscopes came
into use. If the thickness of the coverslip is thinner than the design
of the objective requires, the tube length needs to be increased or
lengthened; if the coverslip is thicker (or the subject is below the
bottom of a standard coverslip), the tube length needs to be shortened.
Modern microscopes do not usually have drawtubes as this is not
feasible for a binocular; one is forced to use an objective with a
correction collar for critical high power work with dry objectives on
such instruments. This is rarely done today; when higher magnification
and resolution are required, the microscopist usually simply resorts to
an oil immersion objective..